Tubulin beta 3 antibody orb44544 from Biorbyt

Product Specs
ItemTubulin beta 3 antibody
CompanyBiorbyt
Price Pricing Info Supplier Page
Catalog Numberorb44544
Quantity0.1 mg
ApplicationsFC, ICC, IHC-P, WB
ClonalityMonoclonal
Conjugate/TagUnconjugated
IsotypeMouse IgG1
HostMouse
TargetbetaIII-Tubulin
ReactivityCanine, Human, Mouse, Porcine, Rat
ImmunogenPeptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide
Concentration1 mg/ml
NCBI Full Gene Nametubulin beta 3 class III
NCBI Gene AliasesCDCBM, CFEOM3A, TUBB4, CDCBM1, FEOM3, CFEOM3, beta-4
PurityPurified by protein-A affinity chromatography
CloneTU-20
StorageStore at 2-8°C. Do not freeze

Specifications/Features

Application Notes: Western blotting: Recommended dilution: 1-2 µg/ml; positive control: porcine brain lysate; negative control: HPB-ALL human peripheral blood leukemia cell line; reducing conditions. Immunohistochemistry (paraffin sections): Recommended dilution: 10 µg/ml, standard ABC technique (DAB+), pretreatment: 0.1% pepsin (trypsin) in 0.1 M HCl; incubation 30 min in RT; or high temperature citrate buffer antigen retrieval; positive tissue: neuronal tissue. Immunocytochemistry: Positive material: Neuro2a mouse neuroblastoma cell line. Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.

Application Dilutions: Western blotting: Recommended dilution: 1-2 µg/ml; positive control: porcine brain lysate; negative control: HPB-ALL human peripheral blood leukemia cell line; reducing conditions. Immunohistochemistry (paraffin sections): Recommended dilution: 10 µg/ml, standard ABC technique (DAB+), pretreatment: 0.1% pepsin (trypsin) in 0.1 M HCl; incubation 30 min in RT; or high temperature citrate buffer antigen retrieval; positive tissue: neuronal tissue. Immunocytochemistry: Positive material: Neuro2a mouse neuroblastoma cell line. Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.

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